Abcam, ab227593, Anti-elF2 alpha (phospho S52) antibody - N-terminal

Size: 100µL
Rabbit Polyclonal EIF2S1 phospho S52 antibody. N-terminal. Suitable for WB and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2S1 phospho S52.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human EIF2S1 phospho S52. The exact immunogen used to generate this antibody is proprietary information.P05198

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eIF2 alpha also known as eukaryotic translation initiation factor 2 alpha is a subunit of the eIF2 complex with an approximate mass of 36 kDa. It plays an important role in protein synthesis initiation by facilitating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. eIF2 alpha is widely expressed across various tissues indicating its fundamental role in cellular function. It is also regulated through phosphorylation as part of its function in modulation of translation initiation.
Biological function summary
EIF2 alpha modulates protein synthesis in response to cellular stress signals. When phosphorylated eIF2 alpha inhibits the formation of the active eIF2-GTP-Met-tRNA complex reducing general protein translation and facilitating expression of stress-response genes. As part of the eIF2 complex it interacts with other proteins like eIF2β and eIF2γ highlighting its role in translation regulation and cellular response to various stimuli. This control mechanism is important for cellular adaptation to changing environmental conditions.
Pathways
EIF2 alpha plays an important role in the integrated stress response (ISR) and the unfolded protein response (UPR). Phosphorylation of eIF2 alpha by kinases such as PERK and PKR links it to these pathways and demonstrates its key position in cellular stress response networks. eIF2 alpha's activity regulates downstream factors including activating transcription factor 4 (ATF4) which mediates the expression of genes involved in the stress response. This interconnectivity with key signaling pathways highlights its regulatory importance.
Dysregulation of eIF2 alpha phosphorylation links to various pathologies including neurodegenerative diseases and cancer. Aberrant eIF2 alpha activity can result in the misfolding of proteins contributing to diseases like Alzheimer's or lead to uncontrolled cell growth observed in certain cancers. In particular relationships between eIF2 alpha and proteins such as tau in neurodegeneration or MYC in cancer pathogenesis highlight its potential as a therapeutic target for these conditions.