Abcam, ab227848, Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal cAMP Protein Kinase Catalytic subunit phospho T197 antibody. Carrier free. Suitable for IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2606Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WB, IHC-P, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes the T197 phospho-site, which is the same site as the T198 on Uniprot P22612.Detects PKA alpha/beta/gamma (catalytic subunit) phosphorylated on threonine 197. Moreover, the antibody will cross-react with PRKX/Y (pT203).

Product details:
ab227848 is the carrier-free version of
ab75991
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein kinase A (PKA) alpha/beta/gamma also known as the PKA catalytic subunit plays a significant role in the regulation of cellular functions through phosphorylation. It has alternate names including phospho PKA and PKA C alpha with an approximate molecular weight of 40 kDa. The PKA catalytic subunit is expressed in various tissues throughout the body. This distribution highlights its broad physiological impact given PKA's involvement in numerous cellular processes.
Biological function summary
PKA alpha/beta/gamma acts as an important regulatory molecule in signal transduction pathways. It functions by phosphorylating serine and threonine residues on target proteins which alters the activity protein interactions or localization of these substrates. PKA functions as part of the cAMP-dependent PKA holoenzyme complex which includes two regulatory (R) and two catalytic (C) subunits. Activation requires the binding of cAMP to the R subunits causing dissociation and releasing the active C subunits to exert their effects.
Pathways
PKA alpha/beta/gamma integrates into the cAMP signaling pathway which modulates numerous physiological processes such as metabolism gene expression and cell growth. In the cAMP pathway PKA interacts closely with proteins like adenylyl cyclase and phosphodiesterases which respectively increase and decrease cAMP levels affecting PKA activation state and subsequent cellular responses. PKA also plays roles within the MAPK/ERK pathway where it intersects with other kinases to influence cell cycle progression and differentiation.
PKA alpha/beta/gamma has connections to cancer and cardiovascular diseases. Mutations or dysregulation of PKA activity can lead to abnormal cell proliferation and survival contributing to tumorigenesis. The catalytic subunit is also linked to cardiovascular disorders through its impact on heart rate and contractility. Interaction with proteins such as AKAPs (A-kinase anchoring proteins) can localize PKA activity affecting local signaling cascades that influence disease pathology.