Abcam, ab229173, Recombinant human HMT protein (Active)

Size: 5µg
Recombinant human HMT protein (Active) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, FuncS, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfers 1.0 nmole of methyl group per minute at 37°C.,
Accession:P50135,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

Product details:
Previously labelled as HNMT.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HMT also known as HMT-H8 or Histone Methyltransferase 8 is an enzyme involved in the transfer of methyl groups to histone proteins an essential step in chromatin modification. It has a molecular mass of approximately 55 kDa. HMT expresses in various tissues with a notable presence in the liver brain and heart tissues. The enzyme's distribution suggests a widespread role in regulating gene expression across different biological systems.
Biological function summary
HMT functions within the context of chromatin remodeling influencing how tightly DNA coiled around histones is therefore impacting gene accessibility. HMT often partners with other proteins to form complexes that precisely regulate methylation status in active transcription sites. By adding methyl groups HMT plays a role in activating or repressing genes affecting a broad range of cellular processes.
Pathways
The enzyme HMT takes part in significant epigenetic pathways such as the histone modification pathway and the gene silencing pathway. It actively interacts with proteins like HDACs (histone deacetylases) within these pathways affecting deacetylation and methylation in chromatin dynamics. HMT's activity often intersects with proteins like DNMTs (DNA methyltransferases) which collaborate to maintain cellular identity through stable gene expression patterns.
Abnormal changes in HMT's activity have been linked to cancer and neurodegenerative diseases. Elevated HMT activity often correlates with certain cancer types where increased methylation leads to the silencing of tumor suppressor genes. In neurodegenerative contexts HMT dysfunction can affect neuronal gene expression contributing to conditions like Huntington's disease. Proteins like p53 and HDAC are often implicated in these disorders alongside HMT as their interactions shift cellular behavior in disease states.