Abcam, ab229195, Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TIE2 phospho Y992 antibody. Carrier free. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1053(N)(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.

Product details:
ab229195 is the carrier-free version of
ab151704
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TIE1 and TIE2 also known as Tyrosine Kinase with Immunoglobulin and EGF homology domains 1 and 2 are receptor tyrosine kinases. TIE1 has a molecular mass of roughly 130 kDa while TIE2 is about 145 kDa. These receptors express mainly in endothelial cells and they localize on the cell surface. Their structure allows them to bind specific ligands leading to signal transduction that influences cell behaviors.
Biological function summary
These receptors play significant roles in vascular development and maintenance. TIE1 and TIE2 form a complex with angiopoietins a group of growth factors important for blood vessel maturation and stability. The interactions between TIE receptors and angiopoietins control processes like endothelial cell survival migration and permeability making them essential for normal and pathological angiogenesis.
Pathways
TIE1 and TIE2 receptors feature prominently in the angiogenesis pathway and the endothelial cell signaling pathway. They interact closely with the angiopoietin family and influence downstream signaling through pathways involving proteins like PI3K/Akt and ERK/MAPK. These pathways are important for regulating endothelial cell functions linking TIE receptors to the maintenance of vascular integrity and response to environmental cues.
Disruptions in TIE1 and TIE2 signaling associate with conditions like cancer and cardiovascular disease. Aberrant TIE2 signaling correlates with tumor angiogenesis and metastasis often involving interactions with VEGF another key angiogenic factor. The dysfunction of these receptors may also contribute to atherosclerosis where improper endothelial cell communication leads to vascular inflammation and plaque formation. Understanding these connections aids in developing targeted therapies for managing such diseases.