Abcam, ab229196, Anti-Tec antibody [Y398] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TEC antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y398,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:ab229196 reacts with an epitope located in the PH domain of Tec.

Product details:
ab229196 is the carrier-free version of
ab32368
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tec also known as Tec protein tyrosine kinase functions as a non-receptor tyrosine kinase involved in signal transduction. Its molecular mass is approximately 72 kDa. Tec is expressed predominantly in hematopoietic cells such as B cells T cells and myeloid lineage cells. This protein plays a significant role in mediating cellular communication by transferring phosphate groups from ATP to specific tyrosine residues on target proteins.
Biological function summary
Tec influences cellular processes like proliferation differentiation and survival. Tec belongs to the Tec kinase family which also includes Btk Itk Rlk and Bmx. Though Tec does not form permanent complexes it interacts dynamically with various signaling proteins. These interactions allow Tec to modulate immune cell development and function significantly impacting the adaptive immune response.
Pathways
Tec is an integral part of the B-cell receptor (BCR) signaling pathway and the PI3K/Akt signaling pathway. Upon activation of BCR Tec aids in signal propagation leading to B cell activation and proliferation. Tec operates synergistically with proteins like Src family kinases leading to the activation of downstream signaling pathways. The protein also facilitates the Akt pathway which controls cell growth and survival by interacting with phosphatidylinositol 3-kinase (PI3K).
Tec links to immunodeficiencies and cancers such as leukemia. Dysfunctional Tec signaling can impair immune responses contributing to conditions like X-linked agammaglobulinemia a disorder often connected to mutations in Bruton's tyrosine kinase (Btk). Overactive Tec signaling may promote leukemogenesis linking Tec to leukemias where it cooperates with proteins such as STAT5 which is involved in cell growth and survival pathways.