Abcam, ab229235, Anti-ATG101 antibody

Size: 100µL
Rabbit Polyclonal ATG101 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ATG101.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ATG101. The exact immunogen used to generate this antibody is proprietary information.Q9BSB4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATG101 also known as Autophagy Related Protein 101 is a critical component of the cellular process known as autophagy. This protein has an approximate mass of 289 amino acids making it a modest-sized protein. ATG101 is expressed in various cell types especially in those with high autophagic activity. It mainly resides in the cytoplasm where it participates in the initiation and regulation of autophagosome formation.
Biological function summary
ATG101 plays an essential role in the maintenance and regulation of cellular homeostasis. It is a member of the ULK1 complex partnering with proteins like ULK1 ATG13 and FIP200. This complex is critical for the initiation step of autophagy helping cells respond to nutrient deprivation by recycling cellular components into essential biomolecules. ATG101 stabilizes the ULK1 complex and enhances its kinase activity important for subsequent steps in the autophagy pathway.
Pathways
ATG101 is involved in autophagy and mTOR signaling pathways. The mTOR pathway regulates cell growth and survival while autophagy ensures the degradation of damaged cellular components. ATG101 interacts with proteins ULK1 and mTORC1 to coordinate the inhibition of autophagy under nutrient-rich conditions and its activation during starvation or stress. This modulation enables the cell to adapt to varying environmental conditions by controlling autophagic flux.
ATG101 has implications in cancer and neurodegenerative diseases. Proper autophagic activity regulated by ATG101 prevents the accumulation of damaged proteins which can lead to diseases. Disruptions involving ATG101 or its associated proteins such as ULK1 are often observed in cancer cells where impaired autophagy can lead to unchecked cellular proliferation. Additionally in neurodegenerative diseases like Parkinson's defects in autophagy-related proteins including ATG101 contribute to neuronal cell death and the accumulation of toxic protein aggregates.