Abcam, ab230017, Anti-DDX11 antibody

Size: 50µL
Rabbit Polyclonal DDX11 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDX11 aa 1-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DDX11 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q96FC9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX11 also known as ChlR1 is a DNA helicase enzyme that belongs to the DEAD/H box family characterized by their Asp-Glu-Ala-Asp (DEAD) motifs. With a molecular mass of approximately 100 kDa DDX11 plays a role in unwinding DNA duplexes which is essential for DNA replication and repair processes. This protein is expressed in various tissues with higher expression levels observed in proliferating cells due to their increased demand for DNA replication machinery. The helicase activity of DDX11 facilitates the resolution of complex DNA structures supporting genomic stability.
Biological function summary
This helicase functions within the cellular nucleus where it assists in chromosome cohesion and accurate chromosome segregation during cell division. As part of the cohesin complex DDX11 contributes to the stabilization of sister chromatid cohesion maintaining proper chromosome alignment. The cohesin complex encompasses several proteins including SMC1 and SMC3 which work collaboratively to ensure correct mitotic and meiotic division. By participating in this complex DDX11 plays an important role in preserving genomic integrity during cellular division and avoiding aneuploidy.
Pathways
DDX11 is integral to the DNA damage response and DNA replication stress pathways. In these contexts it interacts with proteins like ATR (Ataxia Telangiectasia and Rad3 related protein) and Fanconi anemia group proteins coordinating cellular responses to DNA damage and replication impediments. The interaction with ATR emphasizes DDX11's involvement in the ATR-dependent checkpoint signaling pathway enabling cells to delay cell cycle progression in response to genotoxic stress. This coordination helps prevent errors during DNA replication and preserves genome fidelity.
DDX11 mutations and dysregulation have been associated with Warsaw Breakage Syndrome characterized by growth retardation and developmental abnormalities. In this condition the helicase's impaired function leads to defects in sister chromatid cohesion often observed as chromosomal breakage and rearrangements. Moreover abnormalities in DDX11 function can correlate with cancer development as faulty DNA repair and chromosomal instability are common features of tumorigenesis. In such scenarios connections with proteins such as BRCA1 may emerge given BRCA1's role in maintaining genomic stability and DDX11's contribution to DNA repair mechanisms.