Abcam, ab230255, Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag)

Size: 100µg
Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) is a Full Length protein, in the 263 to 670 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for ELISA, WB, SDS-PAGE.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Animal free:No,
Carrier free:No,
Storage buffer:Constituents: PBS

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Eastern Equine Encephalitis Virus (EEEV) E3E2 is a structural protein important for the viral life cycle. E3E2 refers to the heterodimer E3 and E2 proteins. E2 is the larger component with a molecular mass of about 50 kDa. Expressed on the surface of the virus E2 facilitates host cell attachment and entry making it an important player in the infection process. The interaction of E2 with host cell receptors determines the host range of the virus. While E3 assists in the proper folding and processing of E2 it is less understood than E2.
Biological function summary
The E3E2 complex assists the virus in evading host immune responses. The complex is part of the viral envelope that shields the viral genome from host recognition. During viral replication E3E2 facilitates the fusion of the viral membrane with host cell membranes enabling viral entry. This complex is indispensable for the survival and propagation of EEEV within host cells. Although E3 does not get incorporated fully into complete virions it plays a management role in processing E2 emphasizing its importance for viral maturation.
Pathways
E3E2 participates in the viral entry and replication pathways. It functions by interacting with the host cell's surface proteins initiating membrane fusion and subsequent events leading to viral replication. E3E2 interacts with protein markers in the endocytic pathway—critical to viral internalization and uncoating. During EEEV infection E3E2 might also associate indirectly with cellular proteins such as integrins though specific partner proteins in the host pathway remain largely undefined.
E3E2 plays a significant role in the pathogenesis of Eastern Equine Encephalitis (EEE). EEEV causes severe encephalitic disease in both humans and horses with E2 being a primary target for neutralizing antibodies. The virus induces neuroinflammation leading to severe neurological symptoms. Additionally E2 variations can affect virulence and tissue tropism highlighting its role in disease severity. The EEEV infection mechanism involves host proteins such as heparin sulfate which assists E3E2 in mediating cell attachment linking it to the observed clinical manifestations of EEE.