Abcam, ab230344, Anti-MCAF2 antibody

Size: 200µL
Rabbit Polyclonal MCAF2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATF7IP2 aa 300-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ATF7IP2 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.Q5U623

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MCAF2 also known as MBD1-containing chromatin-associated factor 2 or MBD1-interacting protein (MCAF2) is a protein with a mass of approximately 47 kDa. It plays a role in chromatin remodeling by interacting with the methyl-CpG binding domain protein 1 (MBD1). MCAF2 exhibits expression in various tissues with significant levels found in human embryonic kidney cells and fibroblasts. It helps regulate gene expression by recruiting chromatin-modifying enzymes facilitating changes in chromatin structure that can either enhance or hinder transcription.
Biological function summary
MCAF2 functions in the context of DNA methylation and chromatin organization. It is a component of the chromatin-associated complex containing MBD1 that aids in linking DNA methylation status to histone modification important for maintaining gene expression patterns. The function of MCAF2 helps in the organization of euchromatin and heterochromatin regions which is foundational for cellular differentiation processes. MCAF2 collaborates with histone deacetylases (HDACs) to target specific gene loci for transcriptional repression.
Pathways
MCAF2 participates in the regulation of key epigenetic pathways particularly DNA methylation and histone deacetylation. Through its interactions with proteins such as MBD1 and HDACs it contributes to the gene silencing pathways that are important for processes like X-chromosome inactivation and genomic imprinting. MCAF2 plays a role in CpG island methylation pathways influencing the network of proteins involved in transcriptional regulation including those collaborating with histone methyltransferases.
MCAF2 has been implicated in cancers particularly through its involvement in regulating genes associated with cell cycle control and apoptosis. Abnormal expression or function of MCAF2 can disrupt these pathways contributing to tumorigenesis. It also links with neurodevelopmental disorders where disruptions in the epigenetic regulation mechanisms interfere with normal brain development. In both cancer and neurodevelopmental disorders MCAF2 functions closely with MBD1 reflecting a significant interaction in pathogenesis tied to its chromatin remodeling activities.