Abcam, ab230376, Anti-AF10 antibody [EPR20810] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AF10 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20810,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab230376 is the carrier-free version of
ab208016
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The AF10 protein also known as MLLT10 acts mechanically by associating with chromatin to regulate gene expression. The protein weighs approximately 225 kDa. Scientists find AF10 in a variety of tissues prominently in the nucleus where it influences gene regulation through interaction with chromatin structures. As a member of the AF family of proteins AF10 has a basic leucine zipper (bZIP) domain which facilitates its role in DNA binding and transcription regulation.
Biological function summary
AF10 participates in the regulation of gene transcription as part of larger protein complexes. It interacts with other chromatin-associated proteins and contributes to histone modification processes thereby affecting chromatin architecture and transcriptional activity. This binding to chromatin-modifying factors allows AF10 to maintain vital roles in controlling essential genes involved in cell cycle progression and differentiation.
Pathways
AF10 operates primarily in transcription regulation pathways and is linked to the HOX gene regulation pathway. It closely interacts with proteins such as MLL (Mixed-Lineage Leukemia) in the context of these pathways. Through its function in the transcription machinery AF10 facilitates the precise expression of gene sets necessary for proper cell lineage specification and development.
Malfunctions in AF10 are linked to acute leukemias such as acute myeloid leukemia (AML) due to aberrant gene translocations involving the MLL gene. This aberration can result in the creation of MLL-AF10 fusion proteins which contribute to oncogenic processes. Furthermore irregular AF10 expression or mutation can be connected with other hematological disorders impacting hematopoiesis and leading to disease progression. Researchers study its connection with MLL in a bid to develop targeted therapies aimed at modulating these leukemic transformations.