Abcam, ab231535, Anti-ABCB10 antibody

Size: 100µg
Rabbit Polyclonal ABCB10 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB10 aa 450 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Pig,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ABCB10 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9NRK6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab231535 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ABCB10 also known as ATP-binding cassette sub-family B member 10 plays a role as a transport protein involved in the mitochondrial export of molecules. It is a member of the ABC (ATP-binding cassette) transporter family characterized by its ability to move various substrates across membranes using ATP hydrolysis. ABCB10 has a molecular mass of approximately 81 kDa and is expressed mainly in the mitochondria of cells with significant levels in tissues with high metabolic activity like the heart and liver.
Biological function summary
ABCB10 contributes to important cellular functions by participating in heme synthesis. It interacts with other mitochondrial proteins forming complexes that facilitate the import of iron and export of heme precursors. This interaction ensures the maintenance of proper heme levels which are necessary for diverse cellular processes including respiration and detoxification pathways. Its activity is not isolated but connected to broader protein networks within the mitochondrial intermembrane space.
Pathways
ABCB10 participates in the heme biosynthesis pathway which is essential for the production of hemoproteins including hemoglobin and cytochromes. Within this context ABCB10 works closely with proteins such as ferrochelatase which catalyzes the final step in heme production. Another related pathway is oxidative phosphorylation where ABCB10 indirectly sustains energy production by ensuring the availability of heme for cytochrome assembly which is pivotal for electron transport chain function.
ABCB10 plays a significant role in conditions related to mitochondrial dysfunction such as sideroblastic anemia and cardiovascular diseases. Mutations affecting ABCB10 can disrupt normal heme synthesis impacting red blood cell production and leading to anemia. Additionally improper function of ABCB10 influences iron homeostasis which associates with proteins like ALAS2 (aminolevulinate synthase 2) further contributing to erythropoietic disorders and highlighting its role in iron-related pathologies.