Abcam, ab231795, Anti-3-methylcytosine antibody

Size: 50µg
Rabbit Polyclonal 3-methylcytosine antibody. Suitable for Dot and reacts with Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to 3-methylcytosine.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.05% Sodium azide, 0.05% Proclin 300Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
3-methylcytosine also known as 3mC is a modified nucleobase in DNA where a methyl group gets added to the cytosine ring. This process involves methyltransferase enzymes and 3-methylcytosine is smaller in mass compared to other methylated cytosines. Detailed mass data is not always available but it is lighter than 5-methylcytosine. 3mC expresses in diverse tissue types indicating its broad biological relevance. Its formation can influence gene expression and genomic stability therefore impacting cellular function.
Biological function summary
3-methylcytosine contributes to the regulation of DNA structure and accessibility. It does this by affecting the interaction between DNA and protein complexes like chromatin. The presence of 3mC may act as a signal for the recruitment or inhibition of certain proteins involved in DNA repair and transcriptional regulation. While it forms part of complex regulatory networks individual 3mC modifications can have significant impacts on gene activity.
Pathways
3-methylcytosine plays a role in epigenetic regulation mechanisms that control gene expression without altering DNA sequence. One key pathway involving 3mC is the DNA damage response pathway where it can affect the recognition and repair of damaged DNA. Proteins like DNA methyltransferases and TET enzymes interact with 3-methylcytosine reflecting its part in maintaining genome integrity and regulating cellular responses to DNA methylation changes.
Alterations in 3-methylcytosine patterns associate with cancer and neurodegenerative diseases. In cancer aberrant methylation can lead to the silencing of tumor suppressor genes contributing to tumorigenesis. Proteins such as DNMT (DNA methyltransferases) are importantly involved in these epigenetic changes often found deregulated in malignancies. In neurodegenerative disorders unusual 3mC presence can disrupt normal neuronal gene expression potentially linked through pathways involving histone modifications and their regulatory proteins.