Abcam, ab231801, Anti-5-carboxylcytosine (5-caC) antibody

Size: 100µg
Rabbit Polyclonal 5-carboxylcytosine (5-caC) antibody. Suitable for ICC/IF, IP, Dot and reacts with Transfected cell line - Mouse, Nucleic Acid, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to 5-carboxylcytosine (5-caC).
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:IP, ICC/IF, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
5-carboxylcytosine (5-caC) is an epigenetic modification of cytosine involved in DNA demethylation processes. Known also as 5-caC it represents an oxidative derivative of 5-methylcytosine. It usually appears in the genome of mammals. Researchers can detect 5-caC in embryonic stem cells and various tissues that undergo significant cellular differentiation. The exact molecular mass of 5-caC is not generally specified but as a part of a cytosine molecule it contributes to diverse molecular mass of DNA. Scientists use methods like mass spectrometry and antibody-based techniques to measure its presence.
Biological function summary
5-carboxylcytosine plays an essential role in gene regulation and development. It facilitates active DNA demethylation by serving as an intermediate in the TET (Ten-eleven translocation) protein-dependent pathway. Through its interactions it influences the methylation status of DNA impacting gene expression in important developmental stages. 5-caC does not necessarily form a complex independently but works along with TET proteins to remodel chromatin structure and affect transcriptional activity.
Pathways
5-carboxylcytosine functions prominently in the DNA methylation and demethylation pathway. These pathways are key to regulating gene expression and maintaining genomic stability. The TET enzymes convert 5-methylcytosine to 5-caC among other intermediates which then undergo further processing to unmodified cytosine. The involvement of proteins like TET1 TET2 and TET3 demonstrates the interconnected roles within these processes and their impacts on transcriptional regulation.
5-carboxylcytosine displays a significant relationship with cancer and neurological disorders. Aberrant patterns of 5-methylcytosine oxidation and disruption of the DNA demethylation pathway contribute to oncogenesis. The interaction with TET proteins links to several forms of leukemia and brain-related disorders such as Rett syndrome. Through these associations 5-caC serves as an important focus in studying disease mechanisms and potential therapeutic approaches.