Abcam, ab232478, Anti-GNA13 antibody [EPR5436] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GNA13 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5436,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab232478 is the carrier-free version of
ab128900
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNA13 also known as Gα13 is a member of the G12 family of G proteins. It has a molecular mass of approximately 44 kDa and functions as a guanine nucleotide-binding protein. This protein is important in transmitting signals from G protein-coupled receptors (GPCRs) to intracellular signaling pathways. GNA13 is widely expressed in various tissues including endothelial cells smooth muscle cells and the brain. The signaling capability of GNA13 is based on its ability to bind GTP and GDP functioning through a cycle of activation and inactivation.
Biological function summary
The role of G protein subunit GNA13 involves participating in critical cellular functions such as cytoskeletal reorganization cell migration and proliferation. It is an integral part of the G12/G13 subunits` complex which plays a role in controlling cell dynamics and morphology. Through these activities GNA13 contributes to the regulation of processes like embryonic development and tissue repair. Additionally it influences cell behavior through the RhoA pathway affecting actin cytoskeleton and cell shape changes.
Pathways
GNA13 interacts with a variety of important signaling systems. One primary pathway where GNA13 is active is the Rho signaling pathway where it activates RhoA leading to changes in the actin cytoskeleton. This pathway is necessary for cellular processes like adhesion and motility. Additionally GNA13 participates in the mitogen-activated protein kinase (MAPK) pathway influencing cellular proliferation. Through these pathways GNA13 is related to proteins such as RhoGEFs which act as guanine nucleotide exchange factors and MAPKs essential in transmitting mitogenic signals.
GNA13 has implications in cancer development and cardiovascular diseases. Its role in Rho signaling and cell motility can contribute to tumor progression and metastasis with alterations in its function linked to various cancers. Additionally in cardiovascular health GNA13's influence on vascular smooth muscle contraction can relate to conditions such as hypertension. Projecting its influence through RhoA GNA13's malfunction connects it to pathological changes within these disease contexts.