Abcam, ab232549, Anti-TRIM21/SS-A antibody [EPR20290] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TRIM21/SS-A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20290,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This reagent is not recommended for mouse or rat IHC-P and human ICC/IF.

Product details:
ab232549 is the carrier-free version of
ab207728
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIM21 also known as SS-A or Ro52 is a 52 kDa protein that plays a role in the immune system. It functions as an E3 ubiquitin ligase involved in the ubiquitination process. This protein is expressed in various tissues including immune cells like lymphocytes and macrophages. TRIM21 recognizes autoantibodies linked to TRIM21 activity particularly those targeting the SS-A complex. Researchers often use antibodies like anti-TRIM21 or anti-SS-A to study its interactions and effects in cellular processes.
Biological function summary
TRIM21 contributes significantly to the regulation of immune responses and participates in innate and adaptive immunity. It forms part of a complex with other proteins to facilitate the degradation of viral particles through the ubiquitin-proteasome system a process known as antibody-dependent intracellular neutralization. As an important player in the immune defense TRIM21 oversees the timely removal of pathogens and prevents potential overactivation of immune responses that might harm the host.
Pathways
TRIM21 operates within the interferon signaling and NF-κB pathways two important areas of immune response modulation. TRIM21 interacts with molecules like transcription factors that influence the expression of interferon-responsive genes which are critical for pathogen defense. Its role in these pathways highlights its interactions with various immune-regulatory proteins helping to maintain immune system balance and effectiveness during infections.
TRIM21 has strong associations with autoimmune conditions such as systemic lupus erythematosus and Sjögren's syndrome. Autoantibodies against TRIM21 are often used as biomarkers for these diseases and their presence can indicate increased autoimmunity. Relationship with other autoantigens like SS-A/Ro60 further connects TRIM21 with disease mechanisms suggesting its importance in the pathology of these autoimmune disorders. Understanding the role of TRIM21 in these diseases may improve diagnostic and therapeutic strategies.