Abcam, ab232650, Anti-LHFPL5 antibody [EPR16285] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LHFPL5 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16285,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab232650 is the carrier-free version of
ab192242
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LHFPL5 also known as TMHS (Tetraspan Membrane Protein of the Hair Cell Stereocilia) functions mechanically as a component of mechanotransduction in hair cells. This protein has an estimated mass of around 25 kDa and is mainly expressed in the inner ear's cochlear and vestibular systems. It resides in the stereocilia of hair cells where it plays an important role in auditory systems through its association with mechanotransduction channels.
Biological function summary
LHFPL5 contributes to auditory transduction by interacting with other proteins in a complex critical for hair cell function. This complex is involved in converting mechanical stimuli into neural signals. LHFPL5 supports the integrity and function of the mechanotransduction channel allowing the inner ear to detect sound and balance changes. The protein acts as a molecular anchor directly influencing the gating of the channel and overall sensory capabilities.
Pathways
LHFPL5 integrates into the auditory mechanotransduction pathway necessary for hearing. It works in conjunction with proteins such as protocadherin 15 (PCDH15) and myosin 7a which are vital for the linking of molecular structures within the stereocilia. These interactions contribute to the pathway's role in enabling the propagation of electrical signals generated from mechanical excitation fundamental to auditory perception.
Mutations in LHFPL5 are linked to Usher syndrome which causes deafness and progressive vision loss. Additionally its dysfunction is associated with non-syndromic hearing loss. These conditions often involve abnormal interactions with other implicated proteins such as cadherin 23 (CDH23) highlighting the interconnected nature of LHFPL5 within the structural and functional frameworks essential for normal hearing and balance.