Abcam, ab232655, Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RAB11-FIP2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12294-85,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Product details:
ab232655 is the carrier-free version of
ab180504
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB11-FIP2 also known as Rab11 Family Interacting Protein 2 interacts mechanically with the small GTPase Rab11. It plays a role in the transport of recycling endosomes to the plasma membrane. The molecular mass of RAB11-FIP2 is approximately 55 kDa. This protein is expressed in a variety of tissues including epithelial cells and neurons indicating its role in diverse cellular environments. Its function is important for maintaining the dynamics of vesicular trafficking.
Biological function summary
The protein influences endocytic recycling processes facilitating the movement of molecules such as glucose transporters and integrins back to the cell surface. RAB11-FIP2 associates with several other proteins in the Rab11 pathway forming a complex that functions in the coordination and regulation of vesicular trafficking. Its precise regulation of membrane recycling has implications for processes like cell migration and signal transduction.
Pathways
The protein operates within the recycling endosome pathway and is part of the intricate network controlling intracellular trafficking. RAB11-FIP2 has significant interactions with proteins like Rab11 and EHD1. These interactions are integral to the proper functioning of pathways directing the distribution and localization of important cellular components impacting cellular homeostasis.
Improper regulation or mutations of RAB11-FIP2 have been associated with disorders such as cancer and epilepsy. Altered expression levels can affect cellular transport processes contributing to the progression of such diseases. Additionally RAB11-FIP2 shows connections with proteins like GLUT4 in metabolic pathways which suggests a possible role in metabolic disorders highlighting its clinical significance.