Abcam, ab232841, Anti-NR0B2 antibody

Size: 100µg
Rabbit Polyclonal NR0B2 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NR0B2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Pig,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human NR0B2.Q15466

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.011% Proclin 300Constituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NR0B2 also known as small heterodimer partner (SHP) is a nuclear receptor that functions as a transcriptional regulator. It lacks a conventional DNA-binding domain distinguishing it mechanistically from other nuclear receptors. SHP weighs approximately 28 kDa. It is broadly expressed within the liver pancreas spleen and adrenal gland. In these tissues NR0B2 acts by interacting with other transcription factors to repress the transcription of specific target genes influencing various metabolic processes.
Biological function summary
NR0B2 modulates diverse processes such as bile acid synthesis glucose homeostasis and lipid metabolism. It operates alone and does not form part of a complex. Instead it directly interacts with other nuclear receptors and transcription factors including liver receptor homolog-1 (LRH-1) and retinoid X receptor (RXR) to control gene expression linked to metabolism. Through these interactions SHP acts as a vital checkpoint to maintain metabolic stability within the body.
Pathways
NR0B2 is an important regulator in bile acid and gluconeogenesis pathways. Through its interaction with LRH-1 NR0B2 negatively regulates the bile acid synthesis pathway reducing bile acid production when levels are already sufficient. In terms of gluconeogenesis NR0B2 works alongside forkhead box protein O1 (FoxO1) to manage glucose levels in the liver. This control is critical for energy homeostasis responding to the body's nutritional states.
NR0B2 involvement links to metabolic syndromes and non-alcoholic fatty liver disease (NAFLD). Higher or lower levels of NR0B2 influence the development of these conditions by affecting cholesterol and triglyceride storage. SHP's interactions with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) are significant as these can further disrupt lipid metabolism and enhance the risk or severity of these diseases. Understanding NR0B2's role offers pathways for therapeutic interventions focusing on these metabolic disorders.