Abcam, ab233113, Anti-RSPO3 antibody

Size: 100µg
Rabbit Polyclonal RSPO3 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RSPO3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Pig,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RSPO3.Q9BXY4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab233113 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.011% Proclin 300Constituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
R-spondin 3 (RSPO3) also known as CRISTIN3 is a protein with a molecular weight of approximately 27 kDa. Mechanically RSPO3 acts as a regulatory protein involved in modulating the Wnt signaling pathway by facilitating the interaction between LGR receptors and Frizzled LRP6 co-receptors. RSPO3 is expressed in various tissues with high levels observed in the placenta and also in the lung and kidney. This distribution suggests RSPO3 has diverse roles in different physiological contexts.
Biological function summary
RSPO3 functions as a growth factor-like protein that can enhance Wnt signaling leading to the stabilization of β-catenin and promoting cell proliferation and differentiation. RSPO3 is part of a larger protein complex that includes LGR4/5/6 receptors and the Wnt signaling ligands. This interaction is essential in processes like embryonic development and tissue regeneration illustrating its supportive role in maintaining cellular dynamics.
Pathways
RSPO3 plays a significant role in the Wnt/β-catenin signaling pathway where it works closely with proteins such as LRP6 and Frizzled receptors. It also connects with the non-canonical Wnt signaling pathway. This interaction enhances signal transduction vital for cell fate determination. RSPO3’s ability to bind LGR4 and facilitate its role in pathways related to growth and development emphasizes its importance in cellular communication and regulation.
Aberrant expression or mutations of RSPO3 have associations with cancer particularly colorectal cancer through alterations in the Wnt/β-catenin pathway. RSPO3 supports oncogenic processes by enhancing β-catenin stability leading to uncontrolled cell proliferation. It is also relevant in vascular diseases where its interaction with other proteins like VEGFA can influence angiogenesis. Understanding RSPO3’s function in these pathways aids in developing targeted therapies for these conditions.