Abcam, ab233297, Anti-Prothrombin fragment 2 antibody

Size: 100µg
Rabbit Polyclonal Thrombin antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Rat, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F2 aa 1-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Pig,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human F2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.P00734

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.011% Proclin 300Constituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prothrombin fragment 2 also known as F2 fragment is a part of the prothrombin protein that is released during the activation of prothrombin to thrombin. It has a molecular mass of approximately 28 kDa. This fragment appears in the blood plasma specifically produced by the liver cells. In clinical settings the presence of prothrombin fragment 2 can be measured as an indicator of coagulation activity.
Biological function summary
Prothrombin fragment 2 plays a significant role in the blood coagulation process. It is a result of cleavage from prothrombin a glycoprotein that circulates in the plasma. It acts as an important marker for the generation of thrombin which is an active enzyme necessary for fibrin clot formation. The release of the fragment happens during the transformation of prothrombin to thrombin by the prothrombinase complex which includes factor Xa and factor Va.
Pathways
Prothrombin fragment 2 is involved in the coagulation cascade specifically in the common pathway. This pathway is essential for the formation of cross-linked fibrin forming the stable structure of a blood clot. Related proteins include factor Xa which helps activate prothrombin into thrombin and factor Va which acts as a cofactor in the prothrombinase complex highlighting the interconnected nature of the clotting mechanism.
Prothrombin fragment 2 has a correlation with thrombotic disorders and cardiovascular diseases. Elevated levels of this fragment serve as a biomarker for conditions like deep vein thrombosis and atherosclerosis indicating increased thrombin production. Factor Xa another player in the clotting pathway is often targeted in therapeutic interventions for these conditions illustrating the critical nature of understanding the role of prothrombin fragment 2 in disease mechanisms.