Abcam, ab234064, Anti-PDPK1 antibody [3H3D9]

Size: 100µg
Mouse Monoclonal PDPK1 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDPK1 aa 450 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3H3D9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PDPK1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O15530

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDPK1 also known as 3-phosphoinositide-dependent protein kinase-1 or PDK1 is a protein kinase with a molecular mass of approximately 63 kDa. It plays an important mechanical role in the activation of other kinases through phosphorylation on threonine or serine residues. PDPK1 is widely expressed in various tissues including heart lung pancreas and liver reflecting its significant roles in cellular signalling.
Biological function summary
PDPK1 is a master regulator of several AGC kinase family enzymes. It functions as a docking station coordinating with other proteins for activation processes. For example PDPK1 helps in activating PKB/Akt which is required for cell survival and growth. PDPK1 forms part of signaling complexes that manage processes like metabolism growth and survival.
Pathways
PDPK1 plays an essential role in the PI3K/AKT pathway which is an important signaling pathway for cellular growth and survival. This pathway begins with the activation of PI3K leading to the production of PIP3 which creates a binding site for PDPK1 at the plasma membrane where it activates PKB/Akt. Also PDPK1 interacts with NF-κB pathway components linking it to processes in immune response regulation and inflammation.
PDPK1 has associations with cancer and Type 2 diabetes. Abnormal activity of PDPK1 is observed in several cancer types indicating its role in uncontrolled cell growth. In Type 2 diabetes PDPK1 influences insulin signaling disruptions and glucose metabolism. PDPK1 connects these conditions through interactions with proteins like mTOR which influences cell growth and metabolism further exemplifying its importance in disease mechanisms.