Abcam, ab234076, Anti-ARD1A antibody [3G3B9]

Size: 100µg
Mouse Monoclonal ARD1A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, African green monkey, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAA10 aa 100 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3G3B9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human, African green monkey,
Applications:Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NAA10 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P41227

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARD1A also known as NAA10 is a catalytic subunit of the N-terminal acetyltransferase complex. This protein has a mass of approximately 25 kDa. It acetylates the alpha-amino group of nascent polypeptides which affects stability localization and function of proteins. ARD1A is expressed in various tissues including heart skeletal muscle and liver. Its enzymatic activity requires a partnership with NAA15 another subunit which together form the N-terminal acetyltransferase A (NatA) complex.
Biological function summary
Acetylation by ARD1A modulates protein interactions and function. The NatA complex where ARD1A acts influences processes including cell cycle regulation and apoptosis. By modifying substrates critical for cell fate ARD1A plays a role in maintaining cellular balance. This complex helps in modifying proteins like actin which regulates the cytoskeleton a cellular structure essential for movement and division.
Pathways
Protein acetylation by ARD1A fits into the cellular signaling and protein degradation pathways. The complex alters substrates involved in proteasome-mediated degradation relating it to ubiquitin-proteasome pathways. Other interactions include the modulation of gene expression pathways impacting proteins like histones which are core to chromatin structure and gene regulation.
Improper ARD1A function can relate to cancer and neurodegenerative diseases. Alterations in ARD1A activity have connected it to tumorigenesis due to its role in cell growth regulation. Additionally decreased N-terminal acetylation by ARD1A has shown a link to neurodegeneration. This happens as it affects proteins such as tau which is significant in Alzheimer's disease when improperly modified.