Abcam, ab234978, Anti-NAGPA antibody

Size: 100µL
Rabbit Polyclonal NAGPA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAGPA aa 300-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NAGPA aa 300-450. The exact immunogen used to generate this antibody is proprietary information.Q9UK23

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NAGPA also known as N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase plays an important role in the modification of lysosomal proteins. This enzyme's molecular mass is around 62 kDa and it primarily operates within the Golgi apparatus. It is widely expressed across various tissues making it an essential component in the cell's processing and trafficking functions. Other terminology includes hexosaminidase a which reflects its function in cleaving glucosamine residues.
Biological function summary
NAGPA participates in the formation and processing of mannose-6-phosphate recognition markers essential for lysosomal protein targeting. It functions independently rather than as part of a multi-protein complex. The enzyme helps remove a N-acetylglucosamine (GlcNAc) group exposing the mannose-6-phosphate which is a critical step required for lysosomal enzymes to be recognized and transported to the lysosome.
Pathways
Which include NAGPA the enzyme plays a significant role in the lysosomal enzyme targeting pathway and glycoprotein metabolic processes. The mannose-6-phosphate pathway is important for directing enzymes to lysosomes requiring precise interaction between NAGPA and the phosphotransferase proteins responsible for initial phosphorylation. Its regulatory actions ensure lysosomal enzymes are properly targeted and activated.
Malfunction or deficiencies in NAGPA can lead to issues such as mucolipidosis III a disorder characterized by improper lysosomal enzyme targeting. NAGPA's connection with hexosaminidase b involved in the breakdown of GM2 gangliosides also links it to disorders such as Sandhoff disease. This highlights NAGPA's pivotal role in maintaining lysosomal function and contributing to overall cellular health.