Abcam, ab235094, Anti-Bile Acid Receptor NR1H4 antibody

Size: 100µL
Rabbit Polyclonal Bile Acid Receptor NR1H4 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR1H4 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NR1H4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q96RI1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bile Acid Receptor NR1H4 also known as Farnesoid X Receptor (FXR) is a nuclear receptor with a mass of approximately 57 kDa. It functions as a transcription factor regulating the expression of genes involved in bile acid lipid and glucose homeostasis. NR1H4 is expressed mainly in the liver intestines kidneys and adrenal glands where it plays a critical role in maintaining metabolic balance. By binding bile acids NR1H4 activates allowing it to bind to DNA and modulate gene expression.
Biological function summary
NR1H4 influences the body's metabolism by controlling genes responsible for bile acid synthesis conjugation and transport. It forms a heterodimer with Retinoid X Receptor (RXR) to regulate these processes. As an important regulator NR1H4 controls the enterohepatic circulation of bile acids reducing toxicity by lowering hepatic bile acid production. Additionally it influences lipid and glucose metabolism linking it to broader metabolic functions.
Pathways
NR1H4 is involved in the bile acid signaling and lipid metabolism pathways. Within the bile acid signaling pathway NR1H4 modulates the interaction with various proteins such as Small Heterodimer Partner (SHP) to suppress bile acid synthesis. In the context of lipid metabolism NR1H4 interacts with Liver X Receptor (LXR) to regulate cholesterol and triglyceride levels. These interactions demonstrate NR1H4's integral role in maintaining lipid homeostasis.
NR1H4 bears relevance to cholestatic liver disease and nonalcoholic fatty liver disease (NAFLD). In cholestatic liver disease impaired NR1H4 function leads to abnormal bile acid regulation contributing to liver damage. In NAFLD altered NR1H4 activity affects lipid metabolism promoting liver steatosis. Through these diseases NR1H4 shares connections with the CYP7A1 protein critical for bile acid synthesis and the Peroxisome Proliferator-Activated Receptor Alpha (PPARα) involved in lipid oxidation.