Abcam, ab23513, Anti-PKC alpha (phospho S657 + Y658) antibody

Size: 50µL
Rabbit Polyclonal KPCA phospho S657 + Y658 antibody. Suitable for ICC, WB and reacts with Rat samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human PRKCA phospho S657 + Y658.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRKCA phospho S657 + Y658. The exact immunogen used to generate this antibody is proprietary information.P17252,
Specificity:ab23513 detected PKC alpha and PKC beta I, but not other PKC isoforms in rat brain lysate.

Product details:
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PKC alpha known as Protein Kinase C alpha is a serine/threonine-specific protein kinase involved in various cellular processes. The molecular mass of PKC alpha is approximately 80 kDa. Alternately called PKCA or PRKC alpha it is expressed in many tissues with high expression in the brain heart and lungs. PKC alpha plays a role in signal transduction by phosphorylating serine and threonine residues on target proteins.
Biological function summary
PKC alpha regulates cellular functions including proliferation differentiation and apoptosis. It often acts as part of a larger protein complex modulating various biological responses by interacting with substrates and co-factors. Its activity is dependent on calcium ions and diacylglycerol emphasizing its role in calcium and lipid signaling pathways.
Pathways
PKC alpha plays a vital role in the MAPK/ERK pathway and the PI3K/AKT pathway which are critical for the cellular stress response and growth regulation. PKC alpha interacts with several proteins in these pathways including RAF1 and AKT1 facilitating signal transmission that influences cell fate decisions.
PKC alpha has been implicated in cancer and cardiac hypertrophy. In cancers aberrant PKC alpha signaling is linked to increased cell proliferation and survival. In cardiac hypertrophy it is associated with abnormal heart growth due to impaired signaling involving AKT1 and GSK3B. These connections highlight PKC alpha's influence in pathological as well as physiological conditions.