Abcam, ab235338, Anti-PIGX antibody

Size: 100µL
Rabbit Polyclonal PIGX antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIGX aa 1-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PIGX aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q8TBF5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIGX also known as phosphatidylinositol glycan anchor biosynthesis class X functions as a subunit in the glycosylphosphatidylinositol (GPI) anchor biosynthesis pathway. PIGX has a mass of approximately 25 kDa and shows expression in various tissues including the liver and kidney. This protein participates in the early step of GPI anchor formation specifically involved in anchoring proteins to membranes.
Biological function summary
This target is essential in the complex that initiates GPI anchor biosynthesis. PIGX partners with PIG-A PIG-C and other components to catalyze the transfer of N-acetylglucosamine to phosphatidylinositol a critical step in the production of GPI anchors. These anchors tether proteins to the cell surface impacting processes such as signal transduction cell adhesion and protein sorting.
Pathways
PIGX plays a significant role in the GPI-anchor biosynthesis pathway and glycosylation. Within these pathways PIGX closely interacts with other GPI biosynthesis proteins such as PIG-M and PIG-L coordinating the sequential steps necessary for anchor formation. This pathway ensures that proteins anchor properly to cell membranes influencing cellular communication and immune responses.
Defects in PIGX can lead to conditions like paroxysmal nocturnal hemoglobinuria (PNH) and inherited GPI deficiency (IGD). PNH manifests due to mutations affecting GPI anchor synthesis which also involves the PIG-A protein. Additionally issues in the GPI biosynthesis pathway where PIGX is integral can result in neurologic disorders and developmental delays linked to IGD. Understanding PIGX's role aids in diagnosing and developing therapies for these conditions.