Abcam, ab236142, Anti-HES5 antibody [EPR15578] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HES5 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15578,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab236142 is the carrier-free version of
ab194111
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HES5 protein also known as Hairy and Enhancer of Split 5 represents a transcriptional repressor involved in various cellular processes. Having a molecular weight of around 30 kDa HES5 is part of the basic helix-loop-helix (bHLH) family of transcription factors. Its expression predominantly occurs in the nervous system specifically in neural progenitor cells. Additionally HES5 also shows expression in certain tissues during early developmental stages.
Biological function summary
The HES5 protein influences neurogenesis by regulating the transcription of genes involved in developmental pathways. It does not work alone but as part of a larger complex of proteins specifically within the Notch signaling pathway. When active HES5 suppresses the expression of proneural genes thereby maintaining neural progenitor cells in an undifferentiated state an important function for proper neural development. This regulatory role is essential for ensuring the balance between neural progenitor proliferation and differentiation.
Pathways
HES5 functions significantly within the Notch signaling and Wnt pathways which are critical to cellular differentiation and proliferation. The Notch signaling pathway includes interactions with related proteins like Notch receptors and ligands such as DLL1 and JAG1. These interactions modulate gene expression that governs neural stem cell maintenance and differentiation. Within the Wnt pathway HES5 indirectly influences signaling that affects cellular fate decisions showing the protein’s role in developing a cellular environment conducive to tissue repair and regeneration.
HES5 has associations with central nervous system disorders like glioblastoma and neurodegenerative diseases. In glioblastoma altered expression of HES5 contributes to the aberrant regulation of neural progenitor states influencing tumorigenesis. HES5's role in neurodegenerative diseases links to its regulatory control over neurogenesis potentially affecting conditions such as Alzheimer's disease. Connections with other proteins such as NOTCH1 in glioblastoma indicate the complex interplay of HES5 within these disease states.