Abcam, ab236191, Recombinant Diacylglycerol acyltransferase/mycolyltransferase Ag85B protein (Tagged)

Size: 100µg
Recombinant Diacylglycerol acyltransferase/mycolyltransferase Ag85B protein (Tagged) is a Mycobacterium kansasii Full Length protein, in the 41 to 325 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P21160,
Animal free:No,
Carrier free:No,
Species:Mycobacterium kansasii,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Diacylglycerol acyltransferase/mycolyltransferase Ag85B also known as Antigen 85B is an enzyme with a mechanical function that involves the transfer of the acyl group. This protein has a molecular weight of approximately 30 kDa. Ag85B mainly expresses in Mycobacterium species including Mycobacterium tuberculosis. It catalyzes the formation of trehalose dimycolate from mycolic acid and trehalose which are essential components of the mycobacterial cell wall.
Biological function summary
Ag85B plays an important role in the synthesizing of the mycobacterial cell envelope. The enzyme contributes significantly to the cell wall integrity and pathogenicity of Mycobacterium tuberculosis. Ag85B forms part of a complex with other proteins including Ag85A and Ag85C which collectively engage in lipid transfer processes necessary for cell wall biosynthesis. This contributes to cellular processes that are important for the replication and survival of the pathogen within host cells.
Pathways
Diacylglycerol acyltransferase/mycolyltransferase Ag85B stands essential in the mycobacterial lipid biosynthesis pathway. It participates actively in the synthesis of the cell wall that is critical for mycobacterial survival and virulence. Within this pathway it closely interacts with proteins like Ag85A and Ag85C. These relationships are significant within the context of the larger fatty acid synthesis network which highlights the interconnected nature of these enzymatic functions within the bacterial life cycle.
Ag85B is directly linked to tuberculosis through its pivotal role in mycobacterial pathogenicity. This protein emerges as a central target for tuberculosis vaccine development. Its function display connection with other proteins such as Ag85A in forming stronger resistance to host immune responses. Understanding Ag85B's role can advance therapeutic strategies aimed at combating mycobacterial infections.