Abcam, ab236221, Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP66,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab236221 is the carrier-free version of
ab93884
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bcl2 alpha also known as B-cell lymphoma 2 is a protein with a molecular mass of approximately 26 kDa. This protein plays an important role mechanically as a regulator of apoptosis actively inhibiting cell death in various cell types. Expressed in cells of hematopoietic origin Bcl2 alpha localizes mainly within the outer mitochondrial membrane nuclear envelope and endoplasmic reticulum. The protein's prominent function contributes to balancing cell survival and death making it significant in cellular homeostasis.
Biological function summary
Bcl2 alpha controls the intrinsic pathway of apoptosis. It is a part of the Bcl-2 family which consists of proteins that either promote or inhibit apoptosis. Bcl2 alpha interacts with other Bcl-2 family members forming either pro-apoptotic or anti-apoptotic complexes. It ensures cell survival by preventing the release of cytochrome c from mitochondria and interacting with pro-apoptotic proteins like Bax and Bak. This interaction obstructs the activation of caspase enzymes which are critical in the execution of apoptosis.
Pathways
Bcl2 alpha functions within the apoptotic and mitochondrial pathways. In the apoptotic pathway Bcl2 alpha plays an anti-apoptotic role by modulating the activity of pro-apoptotic proteins mainly through its interactions with Bax and Bak. Within the mitochondrial pathway Bcl2 alpha affects the permeability of the mitochondrial membrane thereby influencing the release of factors that initiate caspase activation. These pathways involve interconnected roles with other key proteins these roles affecting processes such as cell cycle regulation and response to cellular stress.
Bcl2 alpha is notably associated with certain cancers and neurodegenerative diseases. In cancers like lymphoma Bcl2 alpha is often overexpressed which leads to increased cell survival and resistance to apoptosis therefore contributing to tumor progression. The protein is also linked to neurodegenerative diseases where it may influence neuronal survival. Connections with proteins like myc in cancers and others in neurological contexts further highlight the importance of Bcl2 alpha in these disorders. Understanding its role offers potential therapeutic targets for treatment strategies.