Abcam, ab236314, Anti-RALA antibody

Size: 100µL
Rabbit Polyclonal RALA antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RALA aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RALA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P11233

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RALA also known as Ras-related protein Ral-A or RalA is a small GTPase involved in a variety of cellular processes. It has a molecular mass of approximately 23 kDa. RALA switches between inactive GDP-bound and active GTP-bound states facilitating the transduction of signals within the cell. This protein is found in a range of tissues with expression in the brain lung and kidney being notable. The ability of RALA to shuttle between membrane compartments highlights its mechanical versatility in cellular signaling.
Biological function summary
The interaction of RALA with effector proteins drives key processes like vesicle trafficking cytoskeletal dynamics and gene expression. RALA forms part of a signaling complex in its active state that mediates these functions. This protein is vital in the neurotransmitter release ensuring efficient communication between neurons. Additionally RALA impacts cell movement by influencing actin filament organization showing its role in cell motility.
Pathways
The engagement of RALA in Ral signaling and the phosphatidylinositol 3-kinase (PI3K) pathway showcases its importance. In Ral signaling RALA interacts closely with proteins like RalBP1 influencing endocytosis and exocytosis activities. In the PI3K pathway RALA helps regulate cell proliferation and survival showing interactions with proteins such as AKT1. These pathways highlight the integration of RALA into broad signal transduction networks important for cellular homeostasis.
The altered regulation of RALA links it to cancer and neuronal disorders. In cancer the dysregulation of RALA can drive tumorigenesis often acting alongside proteins like KRAS in promoting oncogenic signaling. In neuronal disorders improper functioning of RALA has been associated with synaptic dysfunctions potentially affecting proteins like Synapsin I which are important to synaptic vesicle trafficking. Understanding RALA's role in these contexts helps in developing targeted therapeutic strategies.