Abcam, ab236451, Anti-TIPIN antibody

Size: 100µL
Rabbit Polyclonal TIPIN antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMELESS-interacting protein.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human TIMELESS-interacting protein.Q9BVW5

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TIPIN protein also known as Timekeeper Homolog is a 29 kDa protein involved in the regulation of DNA replication and genome stability. It interacts with other proteins to form a complex that plays a role in stabilizing the replication machinery. TIPIN expresses in various tissues including those with high proliferation rates. Researchers study its mechanical role because of its involvement in maintaining the proper functioning of the replication fork a critical part of DNA synthesis.
Biological function summary
The interaction of TIPIN in the replication fork protection complex is important for preventing genomic instability. TIPIN associates with the Timeless protein to form an important complex that maintains the integrity of DNA replication under stress conditions. This complex stabilizes replication forks and prevents DNA breakage allowing smooth progression of DNA synthesis. Without its function cells might encounter failed replication processes and increased mutations.
Pathways
TIPIN integrates into the DNA damage response pathway which maintains genome integrity by activating repair processes. It interacts with the ATR-Chk1 signaling pathway an important mediator of replication stress response. This pathway triggers cell cycle arrest to give cells time to repair damaged DNA. TIPIN collaborates with Timeless and other proteins like MCM helicase preventing replication collapse and maintaining genomic stall points.
TIPIN's role in maintaining genomic stability connects it with cancer development where DNA replication errors can promote tumorigenesis. Changes or mutations affecting TIPIN expression can contribute to breast cancer. Additionally TIPIN is linked to ATR mutations in rare genetic disorders like Seckel syndrome where defective DNA repair causes developmental problems. The relationship between TIPIN and these proteins highlights its possible impact on such diseases serving as a target for therapeutic interventions.