Abcam, ab236466, Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer

Size: 15mL / 60mL / 125mL
Rabbit/mouse dual, HRP/DAB detection IHC kit is a colorimetric micro-polymer assay kit (ab236466) is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). Ideal for studying tissue rich in endogenous biotin e.g. kidney or brain tissue, as ab236466 is a biotin-free immune-enzymatic antigen detection system. - Proven performance: cited in over 65 publications - Smaller detection complex is formed aiding in better tissue penetration - All reagents are ready-to-use as supplied - Available in different sizes to fit your experimental needs
Key facts
Detection method:Colorimetric,
Assay type:Micro-Polymer

Product details:
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit
ab236469
, and HRP-AEC micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit
ab127055
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C