Abcam, ab236502, Anti-ECT2 antibody [OTI2D9]

Size: 100µL
Mouse Monoclonal ECT2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ECT2 aa 150-450.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI2D9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ECT2 aa 150-450. The exact immunogen used to generate this antibody is proprietary information.Q9H8V3

Product details:
Clone OTI2D9 (formerly 2D9).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ECT2 also known as epithelial cell transforming sequence 2 is a guanine nucleotide exchange factor. It has a molecular mass of about 103 kDa. ECT2 is mostly expressed in proliferating cells including tumor cells and plays a role in cell cycle regulation. In its mechanical function ECT2 activates Rho family GTPases especially RhoA by facilitating the exchange of GDP for GTP. This activation is important for the regulation of cytoskeletal dynamics and cell division.
Biological function summary
The regulation of cytoskeletal rearrangement and cell cycle progression involves ECT2. It is an essential part of the mitotic spindle assembly complex. ECT2 interacts with several cellular components to coordinate proper cytokinesis. This activity ensures that the cells divide correctly maintaining proper cell number and tissue homeostasis. ECT2's interaction with other proteins also determines its role in controlling cell shape and movement contributing further to cell cycle events.
Pathways
ECT2 importantly participates in the RhoA signaling pathway which regulates the actin cytoskeleton. The proper function of this pathway is essential for cytokinesis and cell migration. ECT2's activity intersects with proteins like ROCK1 and ROCK2 which act downstream of RhoA to influence cell structure and integrity. In addition to RhoA signaling ECT2 is also involved in the Hippo pathway contributing to cell proliferation controls and tissue growth.
Scientists have found ECT2's involvement in cancer specifically in glioblastoma and breast cancer. Its overexpression in tumors correlates with increased cell proliferation and metastatic potential. In cancer progression ECT2 works with proteins like RhoA and YAP1 in Hippo signaling to influence cell growth and survival making it a target of interest in cancer research and therapeutic development. The aberrant regulation of ECT2 and its pathway partners can lead to uncontrolled cell division and tumor growth.