Abcam, ab236666, Anti-FDFT1 antibody

Size: 100µL
Rabbit Polyclonal FDFT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FDFT1 aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FDFT1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.P37268

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FDFT1 also known as squalene synthase catalyzes the first committed step in cholesterol biosynthesis. This enzyme functions as a dimer and has a molecular weight of approximately 47 kDa. FDFT1 is widely expressed in the liver where cholesterol synthesis primarily occurs but you can also find it present in many tissues throughout the body. As a membrane-bound protein it anchors to the endoplasmic reticulum where it carries out its enzymatic role.
Biological function summary
FDFT1 facilitates the conversion of farnesyl diphosphate to squalene a critical precursor in sterol and cholesterol synthesis. This enzyme does not work in isolation but acts as a part of the larger sterol biosynthesis pathway. The activity of FDFT1 aligns with key metabolic demands particularly in tissues with high rates of cell division or hormone production making it essential for maintaining cellular sterol homeostasis.
Pathways
FDFT1 is an important enzyme in the mevalonate pathway and cholesterol biosynthesis pathway. This enzyme interacts closely with proteins such as HMG-CoA reductase and lanosterol synthase which are pivotal in these pathways. This relationship ensures the proper flow from acetyl-CoA to cholesterol and other isoprenoids critical for cell membrane integrity and signaling functions.
Disruptions in FDFT1 activity can lead to conditions like hypercholesterolemia and related cardiovascular diseases. Excess or deficiency in its function impacts cholesterol levels directly making it a target for cholesterol-lowering therapies. FDFT1's activity also connects to the proteins NPC1 and LDL receptor which play a role in cholesterol transport and uptake further linking it to pathways that contribute to atherosclerosis when dysregulated.