Abcam, ab236758, Anti-GRHL2 antibody [CL3760]

Size: 100µL
Mouse Monoclonal GRHL2 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL2 aa 100-300.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL3760,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GRHL2 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.Q6ISB3,
Epitope:Binds to an epitope located within the peptide sequence VVKAEDFTPV as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRHL2 also known as Grainyhead-like protein 2 homolog serves an important role in transcription regulation. This protein has a mass of approximately 68 kDa. It is expressed in various tissues including the liver kidneys and skin. GRHL2 functions mechanically by binding DNA sequences and influencing the expression of genes involved in epithelial development and maintenance.
Biological function summary
GRHL2 plays a critical role in epithelial cell differentiation and proliferation. It is not known to form part of a larger protein complex but rather acts individually to regulate genes associated with epidermal barrier formation and wound healing. GRHL2 affects keratinocyte behavior and has been shown to modulate cellular adhesion molecules like E-cadherin highlighting its direct impact on cellular architecture.
Pathways
GRHL2 influences key regulatory networks such as the Wnt signaling and epithelial-mesenchymal transition (EMT) pathways. Within these systems GRHL2 interacts with proteins like beta-catenin to modulate gene expression important for maintaining epithelial cell identity. These pathways highlight its label as a gatekeeper against unwanted cellular transition states.
Mutations or dysregulation of GRHL2 have been linked to conditions like breast cancer and hereditary hearing loss. In breast cancer aberrant GRHL2 expression affects oncogenes like MYC facilitating tumor growth and metastasis. In hereditary hearing loss GRHL2's impact on E-cadherin and other junctional proteins leads to inner ear developmental defects shedding light on its role in cellular integrity beyond the skin.