Abcam, ab236906, Anti-RPL27/RPL27A antibody

Size: 100µL
Rabbit Polyclonal RPL27/RPL27A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RPL27.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RPL27.P61353

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RPL27 and RPL27A also known as ribosomal protein L27 and L27A are components of the large ribosomal subunit and weigh approximately 16 kDa. They function mechanically as integral parts of the ribosome which is important for protein synthesis. These proteins occur widely in the cytoplasm of eukaryotic cells contributing to the assembly of ribosomes which are vital for translating mRNA into polypeptides.
Biological function summary
RPL27 and RPL27A function as essential components of the ribosomal machinery that drives protein translation. Both proteins contribute to ribosome biogenesis an important process in cellular protein synthesis. As part of the large 60S ribosomal subunit they participate in the formation and stabilization of the ribosomal structure enabling efficient mRNA translation. These ribosomal proteins work within a complex that includes other ribosomal proteins and ribosomal RNA (rRNA) to facilitate the synthesis of proteins necessary for cell growth and function.
Pathways
RPL27 and RPL27A play significant roles in the translation pathway an essential part of gene expression. They are involved in the initiation elongation and termination phases of protein synthesis. The function of these proteins parallels that of other ribosomal proteins like RPL23 which helps in joining amino acids to form polypeptides. Additionally RPL27 and RPL27A integrate into pathways managing cellular responses to stress by regulating the production of proteins under different conditions such as during nutrient availability or cell proliferation signaling.
Alterations in RPL27 and RPL27A levels or mutations can associate with conditions like Diamond-Blackfan anemia and certain cancers. These proteins have links to oncogenesis as irregularities in ribosome biogenesis can lead to abnormal cell growth and cancer development. In addition their interaction with other ribosomal proteins such as RPL5 in these contexts indicates a broader impact on cellular homeostasis and susceptibility to disease. Understanding the role of these proteins in disease may provide insights into novel therapeutic approaches.