Abcam, ab236976, Anti-PLS1 antibody

Size: 100µL
Rabbit Polyclonal PLS1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLS1 aa 1-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PLS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.Q14651

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PLS1 also known as Plastin-1 or fimbrin acts as a pivotal actin-bundling protein influencing the dynamic restructuring of the actin cytoskeleton. This protein has a known molecular mass of approximately 70 kDa. Within human tissues PLS1 is mainly expressed in the small intestine and inner ear supporting its roles in processes like cellular movement and structural integrity. Studies identify it within the microvillar structures attributing to its function in maintaining microvillar core actin filaments.
Biological function summary
PLS1 influences cellular stability by interacting with actin filaments and linking them into organized bundles. This protein engages within microvilli by enhancing the rigidity and static structure necessary for absorption and secretion processes. PLS1 does not form larger protein complexes but operates intimately with actin. Its bundling ability is essential in maintaining the structural organization of actin-rich cellular extensions like filopodia and microvilli.
Pathways
PLS1 plays an integral role in the regulation of actin cytoskeleton pathways. Its action affects pathways such as the cytoskeletal regulation by Rho GTPases which are vital for cellular shape and movement. Within these pathways PLS1 interacts with actin-related proteins such as cofilin supporting the reorganization and stabilization of actin filament networks essential in cellular morphological changes and motility.
The dysregulation of PLS1 can impact conditions like hereditary hearing loss and intestinal disorders. It is associated with syndromic forms of hearing loss due to its expression in the inner ear where proper actin filament organization impacts auditory function. Additionally PLS1's role in maintaining microvillar structure ties to intestinal pathologies. There is interaction with proteins like myosin VIIA in these contexts where the collaborative function with PLS1 influences microvillus stability impacting hearing-related cellular organization and intestinal absorption efficiency.