Abcam, ab237034, Anti-Pericentrin antibody [EPR21987] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Pericentrin antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21987,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:IHC is recommended for human only.

Product details:
ab237034 is the carrier-free version of
ab220784
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.
Biological function summary
Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.
Pathways
Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.
Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.