Abcam, ab237105, Alexa Fluor® 488 Anti-Ret antibody [EPR2871]

Size: 100µL
Rabbit Recombinant Monoclonal RET antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2871,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ret also known as "rearranged during transfection" is a receptor tyrosine kinase with a mass of approximately 170-175 kDa. Scientists often study Ret because of its key role in cellular signaling pathways. It is primarily expressed in neural crest-derived cells and tissues including the kidneys and the enteric nervous system. Ret is also found in various other cell types throughout the body such as endocrine and hematopoietic tissues.
Biological function summary
The Ret protein facilitates several critical processes within the body. It functions as part of a complex with co-receptors called GFRα (GDNF family receptor alpha) which are required for binding ligands such as GDNF (glial cell line-derived neurotrophic factor). This binding activates downstream signaling pathways that influence cell growth differentiation and survival particularly within the nervous system. The proper functioning of Ret is essential for the development and maintenance of these cellular environments.
Pathways
Ret is an important component of the MAPK/ERK and PI3K/AKT signaling pathways. These pathways mediate various cellular responses including cell proliferation survival and apoptosis. The interactions of Ret with other proteins like SHC and GRB2 within these pathways create ripple effects that impact larger cellular networks. Through these pathways Ret connects with multiple proteins to modulate essential biological processes.
Ret's dysregulation is closely associated with multiple endocrine neoplasia type 2 (MEN2) and Hirschsprung's disease. These conditions arise due to mutations affecting Ret's signaling capabilities. In MEN2 Ret mutations lead to uncontrolled cell growth often resulting in medullary thyroid carcinoma. Hirschsprung's disease on the other hand involves a failure in neural crest cell migration leading to congenital gut motility issues. Researchers explore Ret's interactions with other proteins such as endothelin receptor B (EDNRB) to further understand these diseases.