Abcam, ab23722, Anti-AGE (Advanced Glycation End-products) antibody

Size: 200µL
Anti-AGE antibody (ab23722) is a rabbit polyclonal antibody detecting AGE in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA . - Over 110 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:IHC-Fr, ELISA, WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:In physiological conditions, AGE production is slow and effectively contrasted by anti-oxidant mechanisms. However, under pathological conditions, such as hyperglycaemia, AGE formation is accelerated and exceeds the protective effects of antioxidants, degradative processes and renal excretion(PMID: 34942356). It is recomended using disease or aging-related samples, such as diabetic kidney tissue, aging skin tissue as positive controls.

Product details:
Anti-AGE (Advanced Glycation End-products) antibody (ab23722) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IHC-Fr, IHC-P, WB.
Anti-AGE (Advanced Glycation End-products) antibody (ab23722) has been cited over 119 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-AGE (Advanced Glycation End-products) antibody (ab23722) has high sensitivity and specificity.
Anti-AGE (Advanced Glycation End-products) antibody (ab23722) has 6 independent reviews from customers.
Anti-AGE (Advanced Glycation End-products) antibody (ab23722) is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.15Preservative: 0.05% Sodium azideConstituents: PBS, 0.85% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Advanced Glycation End-products (AGEs) are highly reactive compounds formed when reducing sugars non-enzymatically bind to proteins lipids or nucleic acids. They are often referred to as AGE products or AGE-BSA when in the form of bovine serum albumin (BSA). The molecular weight of AGE compounds can vary significantly due to the diversity of glycation products. AGEs express in various tissues but accumulate more in aging tissues and in certain pathological states. Detection of AGE products often utilizes AGE ELISA assays or fluorescence-based methods to measure AGE-BSA.
Biological function summary
AGE compounds affect cellular and tissue functions by altering structural proteins and increasing oxidative stress. While not a component of a specific complex AGEs catalyze cross-linking of proteins like collagen impacting tissue elasticity. The binding of AGEs to receptors such as RAGE (Receptor for Advanced Glycation End-products) triggers inflammatory pathways and oxidative stress. This interaction fuels the damaging effects of AGEs in biological systems which are assayed through AGE assays.
Pathways
AGE compounds influence several critical biological pathways particularly the NF-kB and MAPK pathways. These pathways mediate inflammation and cellular stress responses. The interaction with RAGE also affects signaling with related proteins like S100/calgranulins which further activate pro-inflammatory responses. The alteration of these pathways by AGE compounds highlights their significance in cellular dysfunction and age-related conditions.
AGE compounds play significant roles in the progression of diabetes and cardiovascular disease. In diabetes AGEs contribute to complications by modifying vascular structure and function where they link with proteins such as HbA1c in glycation. In cardiovascular disease AGE accumulation in vascular tissues enhances vascular stiffness and atherosclerotic lesion formation. The AGE-RAGE interaction perpetuates chronic inflammation further exacerbating these conditions.