Abcam, ab237493, Anti-BUD31 antibody

Size: 100µL
Rabbit Polyclonal BUD31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BUD31.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human BUD31.P41223

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BUD31 also known as Bud Site Selection Protein 31 is a component of the spliceosome machinery and plays an important role in RNA splicing. It weighs about 28 kilodaltons. This protein is expressed in various tissues including the brain and the heart. BUD31 is necessary for proper spliceosome assembly and facilitates mRNA processing by aiding in the removal of introns during pre-mRNA splicing.
Biological function summary
BUD31 interacts with multiple proteins as part of the spliceosomal complex particularly during the catalytic steps of splicing. This interaction is essential for maintaining the fidelity of the splicing process preventing errors that could result in defective or dysfunctional mRNA transcripts. Researchers have observed BUD31 involvement in cellular processes such as cell division and proliferation indicating its importance in cell cycle regulation.
Pathways
Multiple researchers have linked BUD31 to the RNA splicing pathway which is an important step in gene expression. BUD31 also interacts with other spliceosome-associated proteins like SF3B1 highlighting its role in spliceosome assembly and function. Another important pathway is the regulation of the cell cycle where BUD31's influence on mRNA processing affects the expression of genes essential for cell cycle progression. This interaction may influence how cells respond to signals that promote growth or initiate division.
Scientists have linked mutations or dysregulation of BUD31 to cancer. Research suggests that BUD31 impacts the splicing of mRNAs that encode for oncogenes and tumor suppressor genes potentially affecting tumorigenesis. Additionally changes in BUD31 expression could relate to neurodegenerative conditions due to its significant expression in the brain and role in proper mRNA processing. Connections with proteins like BRCA1 in the context of cancer suggest BUD31's influence on cell cycle checkpoints and DNA repair mechanisms.