Abcam, ab237514, Anti-Retinoblastoma binding protein 6 antibody

Size: 100µL
Rabbit Polyclonal Retinoblastoma binding protein 6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBBP6 aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RBBP6 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.Q7Z6E9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The retinoblastoma-binding protein 6 (RBBP6) also known as P2 is a multifunctional protein with a molecular mass of approximately 200 kDa. RBBP6 interacts with various proteins such as p53 and the retinoblastoma protein (Rb). It participates in processes like transcription splicing and ubiquitination. Cells express RBBP6 in tissues across multiple organs with significant levels present in the liver heart and lung tissues. The expression pattern indicates a wide role in cellular functions.
Biological function summary
RBBP6 functions as part of a complex involved in cell cycle regulation and apoptosis. It interacts with the p53 tumor suppressor protein facilitating its regulation and influences various cellular processes. Additionally RBBP6 is implicated in RNA processing due to its association with the 3'-end processing of mRNA. Through these roles RBBP6 ensures proper cellular response to stress and DNA damage connecting it to mechanisms of maintaining genomic stability.
Pathways
RBBP6 plays a significant role in the p53 signaling and ubiquitin-proteasome pathways. Within the p53 pathway RBBP6 modulates p53 activity by affecting its degradation; this interaction impacts cell cycle arrest and apoptosis. It also contributes to the ubiquitin-proteasome system by labeling proteins for degradation which maintains protein quality and turnover. These pathways show RBBP6's pivotal function in controlling cell proliferation and survival.
Anomalies in RBBP6 expression or function link to cancer and retinoblastoma. Mutations in RBBP6 can disrupt its interaction with p53 and Rb proteins leading to uncontrolled cell proliferation and tumorigenesis. The protein's dysfunction may also contribute to the development of retinoblastoma a pediatric eye cancer as improper Rb protein regulation compromises cell cycle control. Understanding RBBP6’s role in these diseases offers potential for targeted therapies in oncology.