Abcam, ab237611, Anti-ATP6V0A1 antibody

Size: 100µL
Rabbit Polyclonal ATP6V0A1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0A1 aa 50-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP6V0A1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.Q93050

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V0A1 also known as a subunit of the V0 domain of the vacuolar ATPase (V-ATPase) is a transmembrane protein involved in the acidification of intracellular compartments. It forms a component of the proton channel within the V-ATPase. This protein weighs approximately 95 kDa and can be found expressed in many tissues such as the kidney brain and liver. Its mechanical role involves contributing to proton translocation across cell membranes which is essential for various cellular activities.
Biological function summary
The protein participates in the ATP-dependent acidification of organelles like lysosomes and endosomes. As a part of a multi-subunit V-ATPase complex ATP6V0A1 helps maintain cellular pH balance and is essential for processes such as protein degradation and receptor-mediated endocytosis. Its activity influences the function of numerous cellular processes including ion transport and neurotransmitter release by regulating the pH in synaptic vesicles.
Pathways
ATP6V0A1 is involved in essential biological pathways such as the endocytic pathway and the lysosomal degradation pathway. Within these pathways V-ATPase in which ATP6V0A1 is a component interacts with several important proteins like ClC-5 which is involved in chloride ion transport. These interactions ensure the maintenance of the acidic environment necessary for enzyme activity and substrate processing within lysosomes and endosomes.
Mutations or dysfunction in ATP6V0A1 associate with pathologies such as osteoporosis and some forms of tumour growth. Abnormal activity of V-ATPase with connection to ATP6V0A1 may influence cancer cell behavior by altering the acidic microenvironment impacting cell invasion and metastasis. Additionally relationship with proteins like mTOR important in regulating cell growth and metabolism highlights its importance in disease development highlighting its potential as a therapeutic target.