Abcam, ab237626, Anti-Themis antibody

Size: 100µL
Rabbit Polyclonal Themis antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human THEMIS aa 500 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human THEMIS aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8N1K5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Themis also known as thymocyte-expressed molecule involved in selection is a protein involved in T-cell development. Themis has a mass of approximately 64 kDa. Researchers have found it expressed specifically in thymocytes a type of immune cell found in the thymus. Mechanically Themis helps in signal transduction processes necessary for the proper development and selection of T-cells which are important components of the adaptive immune system. Themis functions by binding to and modulating various signaling molecules within the immune cells.
Biological function summary
Themis plays a significant role in T-cell receptor (TCR) signaling which is essential for the positive and negative selection of thymocytes. Themis functions as part of a complex that cooperates with other proteins to fine-tune the thresholds for TCR signaling. Through its action it ensures the proper development of functional and self-tolerant T-cells helping to distinguish self from non-self in immune responses. As cells progress through the thymus Themis regulates the strength and duration of signaling ensuring the appropriate intensity for T-cell maturation.
Pathways
T-cell development heavily relies on Themis for its contribution to the TCR signaling pathway. Themis interacts in this pathway with important proteins such as LAT (Linker for Activation of T cells) and SLP-76 which are key components for propagating downstream signaling that leads to T-cell activation. Additionally Themis is involved with the Ras-MAPK signaling pathway which further contributes to cell proliferation and differentiation. Both pathways are critical for maintaining the balance in the immune response allowing for the selection of T-cells that can combat infections while avoiding autoimmunity.
Disruptions in Themis function can link to autoimmune diseases and severe combined immunodeficiency (SCID). Mutations or misregulation of Themis can cause improper T-cell selection leading to the survival of self-reactive T-cells or failure in T-cell development. For example defective Themis activity has connections to autoimmune disorders where the body attacks its own tissues. In such cases proteins like LAT and SLP-76 can also show altered activity which contributes to the development and progression of these immune-related diseases.