Abcam, ab237688, Anti-DHHC-12 antibody

Size: 100µL
Rabbit Polyclonal DHHC-12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC12 aa 100-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ZDHHC12 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.Q96GR4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DHHC-12 protein also known as zDHHC12 functions as a palmitoyl acyltransferase enzyme facilitating the addition of palmitate to target proteins. This post-translational modification of proteins affects their trafficking stability or function. DHHC-12 has a molecular mass of approximately 39 kDa. Expression of DHHC-12 occurs in various tissues with significant levels detectable in brain and some other organs. Its activity frequently involves dynamic regulation of protein interactions and membrane associations.
Biological function summary
DHHC-12 is key in ensuring regulation of protein localization and signaling pathways by mediating protein palmitoylation. As part of a large family of palmitoyl acyltransferases it functions based on substrate specificity displaying selective enzymatic activity towards a subset of protein targets. This selectivity allows DHHC-12 to influence diverse cellular processes including signal transduction and protein trafficking. While not directly part of a multiprotein complex DHHC-12 interacts with various proteins during its palmitoylation activities.
Pathways
DHHC-12 plays important roles in cellular signaling and membrane protein trafficking. It significantly contributes to the Ras signaling pathway which controls cell proliferation differentiation and survival. Through this pathway DHHC-12 interacts with Ras proteins modulating their membrane association and activity. DHHC-12 also influences the Wnt signaling pathway which affects cell fate and tissue homeostasis. It ensures proper membrane localization of Wnt pathway components maintaining the signaling cascade integrity.
DHHC-12 has implications in neurodegenerative conditions with particular relevance to Alzheimer's disease. Altered palmitoylation patterns mediated by DHHC-12 can affect amyloid precursor protein processing or tau protein function linking it to disease pathogenesis. Additionally aberrant DHHC-12 expression or activity correlates with certain types of cancers where it affects important oncogenic pathways. Through these disease associations DHHC-12 connects to proteins such as APP in Alzheimer's disease and oncogenes in cancer highlighting its potential as a therapeutic target.