Abcam, ab238303, Anti-Lamin A + Lamin C antibody [4C11]

Size: 100µg
Anti-Lamin A + Lamin C antibody [4C11] (ab238303) is a mouse monoclonal antibody detecting Lamin A + Lamin C in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4C11,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The 4C11 monoclonal recognizes the Ig-fold domain of lamin A and lamin C (PubMed ID: 20498701).

Product details:
What is this antibody validated in?
Anti-Lamin A + Lamin C antibody [4C11] (ab238303) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of Lamin A + Lamin C?
Anti-Lamin A + Lamin C [4C11] (ab238303) specifically detects a band for Lamin A + Lamin C (UniProt: P02545) at a molecular weight of 74kDa.
Reviewed by scientists
Anti-Lamin A + Lamin C [4C11] (ab238303) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Lamin A + Lamin C antibody [4C11] (ab238303) has been confirmed by Immunocytochemistry testing in LMNA Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [4C11] also available for your convenience: ab238303, Carrier free -
ab244577
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.
Biological function summary
Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.
Pathways
Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.
Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.