Abcam, ab238501, Anti-CRB1 antibody

Size: 100µL
Rabbit Polyclonal CRB1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CRB1 aa 1-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CRB1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.P82279

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CRB1 also known as Crumbs Homolog 1 is a protein with a mass of approximately 160 kDa. Mechanically CRB1 plays a critical role in the structural maintenance of retinal photoreceptor cells. It localizes predominantly to the apical membrane of these cells in the retina. CRB1 helps organize intercellular junctions and maintain cell polarity providing essential support to the retina's architecture and function.
Biological function summary
CRB1 maintains cell polarity and adhesion in photoreceptors which are critical for normal vision function. It is part of the CRB1 complex which includes other proteins such as CRB2 and CRB3. This complex operates within the retina and the brain maintaining the structural integrity necessary for cellular communication and overall tissue organization.
Pathways
CRB1 plays a role in the Hippo signaling pathway and the cellular polarity regulation pathway. In these pathways it interacts with proteins such as MPP5 and AMOT which are integral for regulating cell growth and maintaining tissue homeostasis. These interactions ensure proper functioning and survival of retinal cells preventing their degradation over time.
CRB1 mutations associate with retinal dystrophies including retinitis pigmentosa and Leber congenital amaurosis. These conditions lead to progressive vision loss due to the degeneration of photoreceptor cells. Mutations can lead to dysfunctional interaction with proteins like PALS1 and CRB3 disrupting the cell polarity and junction maintenance which ultimately affects the structural support in the retina.