Abcam, ab238677, Anti-E3 ubiquitin-protein ligase RNF213 antibody

Size: 100µL
Rabbit Polyclonal C17orf27 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNF213 aa 200-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RNF213 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.Q63HN8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
E3 ubiquitin-protein ligase RNF213 also known as mysterin functions as an E3 ubiquitin ligase in the ubiquitin-proteasome system. This large protein has a molecular mass of around 591 kDa. RNF213 mediates the ubiquitination of substrate proteins which marks them for degradation. This enzyme expresses mainly in endothelial and smooth muscle cells with some presence in other tissues. RNF213's role in protein degradation is essential for cellular regulation and protein turnover.
Biological function summary
RNF213 plays a significant function in vascular homeostasis and cellular stress response. It does not typically form part of a larger protein complex but interacts directly with its substrates during ubiquitination. Its activity is important for managing cellular processes linked to cell survival under various stress conditions. RNF213 modifies the stability and activity of proteins involved in these processes which supports proper vascular function and stress management.
Pathways
RNF213 plays a role in the autophagy and hypoxia-response pathways. Through these pathways RNF213 regulates proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and other substrates that are integral to cellular response to low oxygen levels. This regulation contributes to cellular adaptation to stress conditions and affects pathways related to protein turnover and stress signal transduction.
RNF213 mutations associate with Moyamoya disease a rare cerebrovascular disorder and may also relate to some forms of angiogenesis-related pathologies. In Moyamoya disease altered RNF213 affects vascular remodeling and leads to progressive stenosis of the cerebral arteries. Other proteins such as p53 which interact with RNF213 play roles in cellular response to stress indicating possible links to other types of vascular disorders.