Abcam, ab238921, Anti-eIF3B antibody [EPR5804] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EIF3B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5804,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab238921 is the carrier-free version of
ab133601
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eukaryotic initiation factor 3 subunit B (eIF3B) also known as EIF3S9 or p116 plays a central role in the initiation of translation. This protein has a mass of approximately 97 kDa and serves as an important component of the eIF3 complex important for assembling the 40S ribosomal subunit with mRNA and initiator tRNA. eIF3B is ubiquitously expressed in various tissues indicating its fundamental role in cellular protein synthesis.
Biological function summary
EIF3B functions as part of the large eIF3 complex which consists of 13 subunits. This complex is important for the recruitment of mRNA to the 40S ribosomal subunit playing an essential role in the start of translation. eIF3B directly contributes to stabilizing the interaction between mRNA and the ribosome promoting the accurate and efficient formation of the translation initiation complex. This function highlights the importance of eIF3B in regulating cellular protein levels.
Pathways
EIF3B participates in key processes of mRNA translation and is integral to the translation initiation pathway. It interacts with other translation initiation factors including eIF4F and eIF5 orchestrating the assembly and positioning of the ribosome for translation initiation. Moreover eIF3B is also involved in the mTOR signaling pathway linking nutrient sensing to protein synthesis regulation.
EIF3B has been associated with various types of cancer such as breast and prostate cancer where its increased expression correlates with tumor progression and poor prognosis. The dysfunction of eIF3B affects protein synthesis and cell proliferation contributing to oncogenesis. Furthermore eIF3B's role is connected to the dysregulation of mTOR pathways which is significant in cancer development. Its interaction with other proteins like eIF4E and eIF5 signifies its impact on cancer progression pathways.