Abcam, ab238972, Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Caspase-1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 17 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16883,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has low affinity in Ms/Rt brain, H1299 and A549. 55kDa and 80kDa nonspecific bands may be observed in some samples.

Product details:
ab238972 is the carrier-free version of
ab179515
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'Pro Caspase-1' is an inactive precursor of Caspase-1 also known as ICE (interleukin-1β-converting enzyme). It plays an important role in the process of inflammation and apoptosis. The pro-enzyme undergoes proteolytic processing to produce active Caspase-1 which consists of two subunits p10 and p20. The molecular weight of pro Caspase-1 is approximately 45 kDa. It is expressed in various tissues especially in immune cells like macrophages and monocytes. Expression levels can vary according to the inflammatory status of the tissue.
Biological function summary
Pro Caspase-1 is involved in the innate immune response by regulating the maturation of pro-inflammatory cytokines such as IL-1β and IL-18. This protein is a part of an essential multimeric complex known as the inflammasome. The inflammasome facilitates the activation of pro Caspase-1 leading to its conversion to active Caspase-1 initiating the cleavage and release of cytokines that signal the presence of infections and stress in cells.
Pathways
Pro Caspase-1 is integrated into the inflammation and pyroptosis pathways. It interacts with various proteins like ASC (apoptosis-associated speck-like protein containing a CARD) and NLRP3 to form the inflammasome complex which is important for cytokine processing and cell death mechanisms. These pathways demonstrate its significant role in limiting pathogen invasion and resolving inflammation through regulated cellular responses.
Pro Caspase-1 is connected to several inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease. The activity of Caspase-1 regulated by its pro-form contributes to the pathogenesis of these disorders due to excessive inflammation. Additionally abnormal Caspase-1 activation implicates conditions like familial Mediterranean fever which often involve proteins like Pyrin in the inflammatory cascade. Understanding pro Caspase-1 dynamics helps in developing therapeutic strategies to modulate inflammation in these diseases.